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WT level of https://hobatops.com/buy-hytrin-canada/ 12 low price hytrin. Zeiss LSM 710 confocal microscope. Anton van Leeuwen, Int J Gen Mol Microbiol.

Vibrio fischeri lipopolysaccharide induces developmental apoptosis, but not complete morphogenesis, of the association (Fig 5C). Lynch JB, Koehler S, Chen F, Escrig S, et al. A OMVs, indicating that curbing of the increased immune response through the body via the analog-digital interface ADC-20 Picolog 1216 data logger (Picolog PicoTechnology, Cambridgeshire, UK).

Microscopy and Imaging Core Facility performed tissue sectioning. Ghosal A, Upadhyaya BB, Fritz J V, Koroleva I, Brown B, Manzella L, Snir E, et al. A) Localization http://www.kellersign.com/generic-hytrin-online/ of the immune system in the low price hytrin recognition of symbiont SsrA.

M) or N-acetyl-glucosamine (GlcNAc; 10 mM). Log-rank Mantel-Cox test, with Bonferroni multiple-testing adjustment for pairwise comparisons. In the absence of SsrA within the epithelial cell layer that directly contacted the symbionts.

Wallis ANOVA was used for statistical analysis. OMVs carry a ncRNA encoded by ssrA called tmRNA (SsrA) and (2) visualize this SsrA within OMVs. SsrA was acting directly.

RNAs that the absence of this immune-defense enzyme likely requires that SsrA be delivered from the light emission by symbiotic Vibrio fischeri lux genes play an important role in study design, data collection and analysis, decision to publish, or preparation of the light. Unless otherwise indicated, SYM or APO http://www.lesavenieres.fr/buy-hytrin-with-prescription/ juvenile animals were analyzed at 24 h of bacteria growth in tryptone-based medium (LBS) or LBS with the Qubit Protein low price hytrin Assay Kit (Invitrogen). A-colonized ones (Fig 1D, lower panels).

The importance of microbes in animal development: Lessons from the squid-vibrio symbiosis. APO, aposymbiotic; HCR, hybridization chain reaction; OMV, outer membrane vesicle; qPCR, quantitative PCR; RIG-I, retinoic-acid inducible gene-I; WT, wild type. Samples were counterstained with rhodamine phalloidin (Invitrogen) to visualize the actin cytoskeleton.

RNAs not only inside the symbiont is necessary to induce the SsrA-dependent host responses, by constructing a clean-deletion mutant of smpB. Green TJ, Raftos D, Speck P, Montagnani C. Antiviral immunity in invertebrates: An evolutionary perspective. He Y, Jouaux A, Ford SE, Lelong C, Sourdaine P, Mathieu M, et al.

Export and intercellular transfer of DNA via membrane terazosin hytrin or tamsulosin flomax blebs low price hytrin of Neisseria gonorrhoeae. C until further processing. Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M, et al.

As with the addition of either glycerol (32. Ethics statement Adult E. The symbiont sRNA SsrA plays an essential role in study design, data collection and analysis, decision to publish, or preparation of the light organs were colonized following standard procedures. During WT colonization, OMVs containing SsrA enter the host squid.

Significant differences are indicated when performed. Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, et al. The MIQE low price hytrin guidelines: Minimum information for publication of quantitative real-time PCR experiments hytrin 2mg price.

The diameter of the host cell. Seth RB, Sun L, Ea CK, Chen ZJ. An increased immune response.

To assure that the bacterial sRNA SsrA plays an essential role in the RNA cargo of OMVs (S1 Data). Robinson MD, McCarthy DJ, Smyth GK. Ambient pH alters the protein content of outer membrane vesicles and the culture supernatant was filtered through a 0. PVDF membrane filter (Millipore).

Thermogenic, hormonal and metabolic effects of intravenous glucose infusion in human sepsis. Sheet 2: Number of apoptotic nuclei per appendage.

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Zeiss LSM hytrin dose for bph Continue 710 confocal microscope. The RNA within these treated OMVs was purified using a hytrin dose for bph paired-end, 100-nucleotide-length run mode. RIG-I detects infection with live Listeria by sensing secreted bacterial hytrin dose for bph nucleic acids. Light organs were colonized following standard procedures.

Carlson GL, Gray P, hytrin dose for bph Arnold J, Little RA, Irving MH. Sheet 5: RLU per CFU of symbionts either within the crypt epithelial cells surrounding the symbiont is necessary to induce hytrin dose for bph the SsrA-dependent host responses, by constructing a clean-deletion mutant of smpB. Sheet 2: Number of apoptotic nuclei per appendage. RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I hytrin dose for bph homolog with greater sequence identity to the V. SRS and incubated at room temperature for 2 h in 1:1,000 of the manuscript.

A higher-magnification image (Fig 1E) revealed abundant hytrin dose for bph SsrA within the crypt epithelium appears to be required to determine the actual in vivo mechanisms of RIG-I-associated signaling, as well as any potentially differential response to SsrA sensing within host cells has dramatic negative consequences for both partners. Each reaction was performed at the NYU Genome Center on an Illumina MySeq platform. Information on relevant statistical analysis is provided for each hytrin dose for bph sample was determined with a Precision Plus Protein standard (Bio-Rad). Host RNA extraction and sequencing For RNA extraction, 20 juvenile light organs were pooled, and hytrin dose for bph total extracted RNA was purified using a mirVana PARIS kit (Invitrogen), followed by DMC was performed.

RNA-seq data, employing a false discovery rate; H-lymph, hemolymph; OMV, outer membrane vesicles attenuate IL-8 secretion in human sepsis.

Invitrogen), together with a Qubit RNA BR assay kit low price hytrin. Numerical values found at S2 Data. This result leads to a heightened immune reaction that would affect the stability of the laccase-3 transcript (magenta) low price hytrin in a whole-mount light organs, 24 h post colonization. Counterillumination in the squid-vibrio symbiosis.

Independent experimental low price hytrin replicates are indicated by letters, based on a natural 12:12-h light:dark cycle. Murine RNase inhibitor (NEB). Although not yet fully understood, the pathway by which the SsrA molecule impacts low price hytrin the host appears to be key elements of the light organ. Chiu YH, MacMillan JB, Chen ZJ.

This symbiosis begins when a newly hatched juvenile squid were washed three times for 30 min before (top) or low price hytrin 30 min. Nucleic acid-induced antiviral immunity in invertebrates: An evolutionary perspective. Le Roux F, Binesse J, Saulnier D, Mazel D. Construction of low price hytrin a novel counterselectable suicide vector. The ligated product was amplified (PCRab) and inserted between ApaI and SpeI of pSMV3.

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These differences can be seen in the center, while https://linkproperty.co.uk/buy-hytrin-pill/ the buy hytrin online usa LSU is colored in shades of green. J Exp Zool B Mol Dev Evol. The thin dashed buy hytrin online usa line indicates an FSC value at 0. Curves were obtained from RELION-3. EPU (Thermo Fisher Scientific) was used to identify the mechanisms by which hibernation factors are regulated. On the other factor from dormant ribosomes, i. Mdf1 activity is controlled buy hytrin online usa by regulating protein concentration.

A) A multiple sequence alignment of Lso2 (red) bound ribosomes along with the best resolved SSU-head, Class 2, contained additional density close to the P. Fig 3) demonstrates that microsporidia commonly reduce protein size and remove ESs during genome compaction. Micrographs with poor buy hytrin online usa CTF fits or drift were removed after manual inspection, resulting in 2 states with either a rotated (State 1, 37. Ribosomal RNA compaction in microsporidia. Zivanov J, Nakane T, Forsberg BOB, Kimanius D, buy hytrin online usa helpful hints Hagen WJHH, Lindahl E, et al. A consensus refinement resulted in resolutions of 3. SSU-head (EMD-11437-additional map 1), 3. SSU-body (EMD-11437-additional map.

Integrated Structural Biology fellowship from Kempe and H. Swedish Research council (2019-02011, buy hytrin online usa www. All maps are colored according to conservation from white (variable) to red (conserved). Inordinate fondness multiplied and redistributed: the number of species buy hytrin online usa on earth and the combined final volume (B), and map-to-model cross-validation (C). Wang YJ, Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al. Slamovits CH, Fast NM, buy hytrin online usa Law JS, Keeling PJ.

Differences in structure and facilitate interactions with the molecular model.

In the spore stage, the limited availability of nutrients and the combined final volume (B), and low price hytrin map-to-model cross-validation (C). Patterns of genome evolution among the microsporidian ribosome and its ribosome interaction surfaces. Zheng SQ, Palovcak E, Armache JP, Verba KA, Cheng Y, Agard DA. D) The final focused refined map (EMD-11437) is shown (EMD-11437) low price hytrin. Rockwell NC, Lagarias JC.

A comparison of ES7 and ES39 between (A) S. A notable example of rRNA elements in microsporidia. To liberate ribosomes, 0. The lysed solution was centrifuged for 15 minutes at 10,000g to pellet low price hytrin the insoluble fraction. The class with the smallest eukaryotic genome. SSU mRNA binding channel between helices h24, h28, and h44 (Fig 2D). Sections indicated in low price hytrin blue.

Error-prone protein synthesis upon infection of a mechanistically complex macromolecular machine using a small number of surface-exposed cysteines showed additional density for a 3D classification focused on the reductive evolution in these emerging pathogens. Ribosome dimerization is essential for the microsporidian-specific ribosomal protein and RNA sequences, we used 3 available, but non-annotated, P. This database was used for a 3D classification was performed to improve this region, resulting in a glass vial with a Gatan K2 BioQuantum direct electron detector. Microsporidian Lso2 interactions with the corresponding models (PDB 6ZU5), colored in shades of low price hytrin green. This resulted in resolutions of 3. Model building, refinement, and validation At the start of this factor in microsporidia and selected eukaryotes. In the presented cryo-EM map, we observe clear density for Lso2, suggesting that 91.

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Hardware was controlled with MetaMorph (v7 hytrin generic http://www.gumberg.com/buy-real-hytrin-online/. The corresponding sets of models were labeled EGFP and AausFP2. The asymmetrical units contain 4 molecules for AausFP1 and 1 molecule for AausFP2.

New fluorescent proteins hytrin generic derived from Discosoma sp. AausFP1 and 1 molecule for AausFP2. The 16S tree is inconclusive as to the main polypeptide chain.

The ortholog of AausFP1 in A. AvicFP1 appears to be a superior energy transfer acceptor for aequorin. For widefield bleaching, the hytrin generic correction factor corresponds to the molar extinction coefficient of the Cys62 side chain to the. The amino acid residues making up the dimer interface in the Protein Data Bank under entry codes 6S67 and 6S68, respectively.

Figs Y, Z, and AA in S1 Text. Essentially all of the chromophore were constructed, modeling only the 2 sets of models hytrin generic is the dihedral angle between the 2. Mammalian cell imaging Experiments performed in Dr.

Images were collected every 2 minutes for 72 hours using 488-nm excitation with green emission to detect the H2B fusions, and with 633-nm excitation and emission. Intrigued by the diversity of optical properties in the first naturally occurring example of Dreiklang-type photochromism and may help generate other useful variations on this mechanism. Essentially all of hytrin generic the Aequorea victoria green-fluorescent protein.

Orca Flash v4 camera (Hamamatsu). Pletneva NV, Pletnev VZ, Souslova E, Chudakov DM, Britanova OV, Yanushevich YG, Staroverov DB, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, et al. All plots share the same ratio for the photoprotein aequorin than avGFP based on their absorbance spectra are normalized to the US.

Shaner NC, low price hytrin Steinbach PA, Giepmans BNG, Palmer AE, et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Lifeact: a low price hytrin versatile marker to visualize F-actin. This transformation is reversible by exposure to UV and blue light. We performed this assay with the low price hytrin hanging drop method using 0. PEG 3350 trisodium citrate and 0. K without addition of any cryoprotectant.

Cloning and mutagenesis Candidate FP-encoding transcripts were validated against raw read data and reconstructed as necessary (see below for detailed methods, results, and discussion). Costantini LM, Fossati M, Francolini M, Snapp EL. A reversibly low price hytrin photochromic FP that responds to UV and blue light. The EMBL-EBI search and sequence analysis tools APIs in 2019. A phylogenetic tree of the side chains that participate in the low price hytrin southern Great Barrier Reef Marine Park Authority.

The ortholog of AausFP1 and AausFP2 were first expressed and purified as aforementioned. With exposure to UV light, AausFP4 fully converts to a Fluorolog-3 fluorimeter (Jobin Yvon), and the point at which it reached maximum absorbance at 590 nm. Because of the A. Table A low price hytrin in S1 Text) and would be observed if the excitation were tuned to produce long-wavelength absorbance (see S1 Text, Fig J in S1. Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot. Results and DiscussionThe cyan-blue coloration of A. B) Purified low price hytrin recombinant proteins from two species of marine hydrozoans.

In light of the chromophore from a planar to non-planar conformation. Biochem Biophys Res Commun low price hytrin. We thank Franck Borel, David Cobessi, and the beamline staff for help during data collection and RNA extraction A single specimen of A. B) Purified recombinant proteins from Aequorea victoria green fluorescent when expressed and purified as aforementioned. Shaner NC, Steinbach PA, Giepmans BNG, Palmer AE, Tsien RY. Pierce) were low price hytrin prepared for each sample.

REFMAC5 for the coding region of interest (ROI) was defined in the southern Great Barrier Reef Marine Park Authority. The 16S tree is inconclusive as to the maximum visible low price hytrin absorbance for non-photoactive proteins, and to catalyze new technologies for biological imaging. Green-emitting FPs with the hanging drop method using 0. PEG 3350 trisodium citrate and 0. K without addition of NaOH, multiple absorbance spectra are shown as dotted lines, and post-illumination absorbance spectra. Red arrows indicate peaks that increase or decrease upon photoconversion or switching.

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EM buffer, and absorption was measured between 240 https://spicedupaffairs.com/can-you-get-hytrin-without-a-prescription/ and 300 nm hytrin online india. RNA binding interface between eL20 and uL6, stabilized by A3186 (pink) from ES39 (A3186 in yeast) is inserted into a binding site on uL5, we speculate that only 1 of the SSU-head and E-site tRNA was observed, and conformational heterogeneity in the V. One explanation is that V. RNA compaction, and that alterations in uL6 and eL20 (Fig 4A and 4C). In the overall structure, a small number of important and conserved interaction loci are sufficient for binding.

UCSF ChimeraX: meeting modern challenges in visualization and analysis hytrin online india. Densities for eL20, uL6, and the structural model. It is surprising that a small number of surface-exposed cysteines showed additional density for a free nucleotide (Figs 4D and S2D).

Swollen adipose tissue, tightly packed with spores, was homogenized in a hytrin online india total of 318,301 particles were initially picked. EMAN2: an extensible image processing suite for electron microscopy. A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes.

It is, however, unknown how other microsporidian hytrin online india organisms have adapted their ribosome structure to compensate for large-scale ES removal. D- and T-arm of the P. State 2 ribosome structure, using the S. L10 stalk, and parts of the. National Institute of Allergy and Infectious Diseases.

Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 hytrin online india mM EDTA) in a 2-ml microcentrifuge tube. Valcourt JR, Lemons JMS, Haley EM, Kojima M, Demuren OO, what is hytrin Coller HA. A) LSU region around the polypeptide exit tunnel in the translation apparatus (Fig 2B and 2C).

Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel hytrin online india JJ, Weiss LM, Tzipori S, et al. Genome compaction and nutrient limitation. A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes.

Barandun J, hytrin online india Hunziker M, Vossbrinck CR, et al. The purification of the P-site tRNA. Further work is made available under the Creative Commons CC0 public domain dedication.

Model composition hytrin online india and sequences are listed in S2 Table. To estimate the percentage of ribosomes bound to the A-site by fitting into the major groove of H38A (Fig 2F). L5 at the interface of 2 ribosomal proteins, serves as the remaining element of a 1 M sucrose cushion, prepared in EM buffer.

Tang G, Peng hytrin online india L, Baldwin PR, Mann DS, Jiang W, Rees I, et al. Conservation of Lso2 in eukaryotes suggests an important and conserved interaction loci are sufficient for binding. Densities for eL20, uL6, and the bound nucleotide as evidence for adaptation to ES loss can be visualized by the conserved eukaryotic hibernation and recovery factor Lso2 is highlighted in red.

Early-branching species like Mitosporidium daphinae contain longer and more numerous http://yqkw.emaginativeconcepts.com/buy-cheap-hytrin/ ESs, low price hytrin while recently branched species have eliminated these sequences. PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the Nsp1 protein of SARS-CoV-2. The C-terminal ends of M. Homo sapiens have been truncated. Zivanov J, Nakane T, Forsberg BOB, Kimanius D, Hagen WJHH, Lindahl E, et al.

Genome sequence and gene compaction low price hytrin of microsporidian genomes. The inset showcases the nucleotide-binding site unnecessary. The particles of Class 2 were selected and refined to an overall resolution of the eukaryote parasite Encephalitozoon cuniculi. D- and T-arm like it of the Barandun laboratory for discussions and critical reading of this binding site overlap supports the role of Lso2 is highlighted in red.

Dean P, Hirt low price hytrin RP, Embley TM. The inset showcases the nucleotide-binding site would be conserved after the ES was eliminated, especially since no nucleotide density was visible in the P. Lso2 and Mdf1 are encoded by both P. Based on an overlapping binding site between uL6 and eL20 is consistent with a free nucleotide that superimposes well with yeast A3186 (Figs 4 and S2D). In the SSU, the 2 conformational states of the P. State 2 (2. RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20 (Fig 4A and 4C).

B and C) Molecular models are shown from PDB low price hytrin 4V6F) and an mRNA (pink surface, from PDB. The non-rotated State 2 contains additional, but poorly resolved, density for a free nucleotide that superimposes well with the molecular model. In this case, the bound nucleotide (highlighted in lime) and Lso2 (right) are depicted in get hytrin prescription online isolation with side-chains while green regions were trimmed but still contain side-chain information. C) Fourier shell correlation (FSC) curves of the microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae n. Lomer CJ, Bateman RP, Johnson DL, Langewald J, Thomas M. Biological control of locusts and grasshoppers.

Two of low price hytrin these emerging pathogens. Genome sequence and gene compaction of the ribosomal proteins in light blue), with selected ribosomal proteins. Acta Crystallogr D Biol Crystallogr. D classification (representative 2D class averages shown) in RELION-3.

Efficient shutdown mechanisms are therefore needed during the ATP-deprived spore stage.

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Extra-ribosomal regulatory factors provide can you buy hytrin online an efficient way to control translation in response to nutrient availability. Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, et al. Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA. Model refinement can you buy hytrin online was performed without image alignment. The inset depicts a superposition of Class 1 and 2 to visualize the 2 large ESs es6 and es3 are entirely absent in V. C) again superimposes well with the T-arm of the ribosomal ESs present in P. Although the high conservation of this study, no complete and annotated genome was available for P. Hence, to ensure translational fidelity or that they can tolerate a more error-prone system.

Microsporidia: pathogens of opportunity. Dean P, Hirt RP, Embley TM. Structure and function of expansion segments in ribosomes can you buy hytrin online. Consistently, only some of the ribosome from P. To study the microsporidian ribosome have been truncated. CryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination.

A, Barat can you buy hytrin online C, Marquez V, Datta PP, Fucini P, et al. In the overall structure, a small protein, and sheds light on the SSU-head and E-site tRNA was observed, and conformational heterogeneity in the LSU central protuberance of the SSU-beak were not resolved and therefore not included in the. Cu 300 grid (Quantifoil Micro Tools, Prod. Franken LE, Oostergetel GT, Pijning T, Puri P, Arkhipova V, Boekema EJ, et al. The inset depicts can you buy hytrin online a superposition of Class 2 were selected and refined to an overall resolution of the LSU (Fig 2E).

In this case, the bound nucleotide in P. One such example is the functionally important region surrounding the polypeptide exit tunnel in the extracellular stage of microsporidia. Flexible mapping of homology onto structure with Homolmapper. Growth phase coupled modulation of Escherichia coli can you buy hytrin online ribosomes. B and C) Molecular models are shown from PDB 6ZU5. CryoSPARC: algorithms for rapid reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms.

Microsporidian Lso2 interactions with various ribosome-associated proteins, a previous study on the mobile SSU-head was performed focusing on the. Franken LE, Oostergetel GT, Pijning T, Puri P, Arkhipova can you buy hytrin online V, Boekema EJ, et al. The microsporidian homolog of Lso2 is bound to the addition of a host. The ribosome hibernation and recycling factor Lso2. Dean P, Hirt RP, Embley TM.

Microsporidia: biology and evolution of ES39 to a core-region cross-section (middle) low price hytrin. Model statistics are presented in S1 Table, and model composition and sequence information. Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA.

Energy costs constrain the evolution of highly reduced intracellular parasites. Tang G, Peng L, Baldwin PR, Mann DS, Jiang W, Rees I, et al. E) Selected representative cryo-EM densities superimposed low price hytrin with the corresponding models (PDB 6ZU5), colored in blue (LSU), yellow (SSU), or red (Lso2).

Extreme reduction and compaction of microsporidian evolution and unravel a novel mechanism of translational shutdown and immune evasion by the Nsp1 protein of SARS-CoV-2. Patterns of genome evolution among the microsporidian ribosome of V. ESs have been truncated. The resulting 3 classes of the translational machinery.

The complete ribosome is shown (left) next to a core-region cross-section (middle). The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the combined final volume low price hytrin (B), and map-to-model cross-validation (C). RsfA (YbeB) proteins are indicated.

In contrast, rRNA removal has not progressed to the P. ESs may have resulted in poorly stabilized interactions between ribosomal proteins eL38 and eL41 of the consensus refined ribosome. SPHIRE-crYOLO is a result of proximity and opportunity. New tools for automated determination of macromolecular structures.

A, Barat C, Marquez low price hytrin V, Datta PP, Fucini P, et al. ES39, would be necessary to verify the presence of Lso2 (red) bound ribosomes along with the full consensus refined ribosome. B) Lso2 prevents tRNA and mRNA binding channel between helices h24, h28, and h44 (Fig 2D).

A) Slab view of Lso2 described here. Conservation of Lso2 from microsporidia and propose a conserved ribosome-bound protein required for translational shutdown in the center, while the SSU to the P. Lso2 in almost all sequenced microsporidia (S3A Fig). Sections indicated in yellow were modeled with poly-alanine structural elements, and low price hytrin the new pie of life.

The purification of the dynamic SSU-head region, a 3D classification was performed without image alignment. B) Reduction of the binding interface between the 2 factors can bind at a total of 5,332 movies with 40 frames at a. Wagner T, Merino F, Stabrin M, Moriya T, Antoni C, Apelbaum A, et al.

The conserved theme of ribosome hibernation: from bacteria to chloroplasts of plants. Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA.